Table 1.
Antibodies used in this study.
| Ab clone | Staining pattern | Host description | Background | Ab dilution pre-treatment | Supplier cat. no. |
|---|---|---|---|---|---|
| Beta III tubulin (TUBB3) | Cytoplasmic | Rabbit polyclonal | β-Molecule of microtubules-neuron specific marker (Molea et al., 1999) | 1:1500 CC1-standard | Abcam Cambridge, UK ab 18207 |
| Peripherin (PRPH) 3B3 | Cytoplasmic | Mouse monoclonal | Type III neuronal intermediate filament (Liu et al., 2010; Barclay et al., 2011) | 1:400 CC1-standard | Lifespan Seattle, WA, USA LS-B6138 |
| MAF B polyclonal | Nuclear | Rabbit polyclonal | Basic leucine-zipper containing transcription factor family; transcription factor that is expressed in (Choo et al., 2006; Marrs and Spirou, 2012) | 1:600 CC1-standard | Sigma-Aldrich St. Louis, MO, USA HPA005653 |
| Synaptophysin YE269 | Nuclear | Rabbit monoclonal | Presynaptic vesicle protein p38 in neuronal and endocrine cells (Khalifa et al., 2003) | 1:600 CC1-standard | Abcam ab 32127 |
| S 100 4C4.9 (Raised against ß-chain) | Cytoplasmic | Mouse monoclonal | Calcium binding protein (Liu and Rask-Andersen, 2014) | ready to use | Ventana Roche Mannheim, Germany 790-2914 |
| PAX2 | Nuclear | Rabbit polyclonal | Paired box gene 2 transcription factor (Fekete and Wu, 2002) | 1:35 CC1 standard | Invitrogen, Camarillo, CA, USA 18-0483 |
| PAX6 | Nuclear | Rabbit polyclonal | Paired box gene 6 transcription factor (Xu et al., 1999) | 1:18 CC1 standard | Sigma Life Sciences, St Louis, MO, USA HPA030775 |
| PAX8 | Nuclear | Mouse monoclonal | Paired box gene 8 transcription factor (Lotan et al., 2009) | ready to use | Ventana Roche Mannheim, Germany 760-4618 |
| Ki-67 (30-9) | Nuclear | Rabbit monoclonal | Proliferative activity of normal and neoplastic tissue (Takebayashi et al., 2005) | ready to use CC1-standard | Ventana Roche 790-4286 |
| Cleaved caspase 3 (Asp 175) | Nuclear | Rabbit polyclonal | Marker for apoptosis (Gown and Willingham, 2002) | 1:400 CC1-standard | Cell Signalling Boston, MA, USA 9661 |
| Glutamine synthetase polyclonal | Cytoplasmic | Rabbit polyclonal | Catalyses the production of glutamine and GABA (Eybalin et al., 1996; Procacci et al., 2008) | 1:10000 CC1 standard | Sigma-Aldrich G2781 |