Table 1.
Details of proteins identified by mass spectrometry.
| Spot No. | Accession number | Protein identified | MASCOT score | Nominal mass (Da) | pI | Sequence coverage % | ORF No. | Densitometric ratio of protein intensity between sensitive and resistant isolates | Functional category* |
|---|---|---|---|---|---|---|---|---|---|
| D 1 | P9WG55 (TIG_MYCTU) | Trigger factor | 78 | 50586 | 4.47 | 33 | Rv2462c | 1: 3.80 | 2 |
| D 2 | P9WQD7 (FAB2_MYCTU) | 3-oxoacyl-[acyl-carrier-protein] synthase II | 133 | 44250 | 5.27 | 35 | Rv2246 | 1: 2.50 | 7 |
| D 3 | O53665 (O53665_MYCTO) | 3-ketoacyl-ACP reductase | 198 | 46988 | 5.86 | 62 | Rv0242c | 1: 2.60 | 7 |
| D 4 | Q79FN7 (Q79FN7_MYCTU) | Transcriptional regulator MoxR1 | 117 | 40752 | 5.96 | 36 | Rv1479 | 1: 2.20 | 4 |
| D 5 | P9WQA3 (ALF_MYCTU) | Fructose-bisphosphate aldolase | 74 | 36522 | 5.49 | 29 | Rv0363c | 1: 3.50 | 1 |
| D 6 | P9WFD7 (Y2623_MYCTU) | Universal stress protein | 70 | 31632 | 5.46 | 41 | Rv2623 | 1: 1.83 | 5 |
| D 7 | P64831 (Y1360_MYCTU) | Uncharacterized Protein | 68 | 37570 | 6.38 | 27 | Rv1360 | 1: 2.50 | 1 |
| D 8 | P9WQB7 (AHPC_MYCTU) | Alkyl hydroperoxide reductase subunit C | 142 | 21553 | 4.50 | 83 | Rv2428 | 1: 2.90 | 5 |
| D 9 | P96907 (P96907_MYCTX) | Enoyl-coA hydratase | 79 | 24281 | 5.52 | 57 | Rv0632c | 1: 3.98 | 7 |
| D 10 | P9WKF5 (KAD_MYCTU) | Adenylate kinase | 168 | 20113 | 5.02 | 72 | Rv0733 | 1: 3.60 | 1 |
| D 11 | P9WGD5 (SSB_MYCTU) | Single stranded DNA-binding protein | 86 | 17343 | 5.12 | 40 | Rv0054 | 1: 2.10 | 3 |
| D 12 | P9WHW3 (PPIA_MYCTU) | Peptidalprolyl isomerase | 86 | 19271 | 5.80 | 54 | Rv0009 | 1: 2.80 | 3 |
| D 13 | P9WHW3 (PPIA_MYCTU) | Peptidalprolyl isomerase | 89 | 19271 | 5.80 | 54 | Rv0009 | 1: 3.10 | 3 |
| D 14 | P9WPJ7 (MTCA1_MYCTU) | Beta carbonic anhydrase 1 | 117 | 18177 | 5.48 | 45% | Rv1284 | 1: 2.81 | 1 |
| D 15 | P9WFN1 (Y2140_MYCTU) | UPF0098 protein | 137 | 18622 | 5.41 | 55 | Rv2140c | 1: 1.80 | 6,1 |
| D16 | O53519 (O53519_MYCTU) | Hypothetical protein | 74 | 16314 | 4.75 | 45 | Rv2185c | 1: 1.90 | 6,7 |
| D17 | P9WFC9 (Y1636_MYCTU) | Universal stress protein | 69 | 15303 | 5.51 | 44 | Rv1636 | 1: 1.72 | 5 |
| D 18 | P9WMK1 (ACR_MYCTU) | Alpha-crystallin | 63 | 16217 | 5.00 | 48 | Rv2031c | 1: 1.83 | 5 |
| D 19 | P9WP75 (CSPA_MYCTU) | Probable cold shock protein A | 51 | 7366 | 5.17 | 83 | Rv3648c | 1: 2.60 | 5 |
| D 20 | Q6MWZ8 (Q6MWZ8_MYCTU) | Conserved Hypothetical protein | 134 | 9884 | 4.86 | 90 | Rv3208A | 1: 1.90 | 6 |
1, intermediary metabolism and respiration; 2, cell wall and cell processes; 3, information pathways; 4, regulatory proteins; 5, virulence, detoxification adaptation; 6, conserved hypothetical; 7, lipid metabolism.
*
Note on functional category.