Figure 2.

Z-ring reassembly upon FtsW reinduction. Spores of strain PG80 (P_spac-ftsW; amyE::P_xyl-gfp-ftsZ) were heat shocked as described and germinated at 37°C in the absence of IPTG. FtsW was reinduced by adding 1 mM IPTG 5 h after the beginning of germination. (A) Phase contrast and GFP images taken every 30 min after re-induction. Scale bar indicates 5 μm. (B,C) Analysis of the relative positions of Z-rings in FtsW depleted filaments (B) and 30 min after the re-induction of ftsW expression (C), determined using a combination of phase and fluorescence images. In both cases, the positions of the detected Z-rings have been expressed as the distance from the nearest cell pole and as a percentage of the cell's total length.