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. 2016 Nov 15;7:1808. doi: 10.3389/fmicb.2016.01808

Figure 3.

Figure 3

The localization of PBP 2B and FtsL depend on FtsW. (A,B) Localization of PBP 2B in FtsW depleted cells and germinating spores. (A) Strain PG87 (Pspac-ftsW; Pxyl-gfp-pbpB) was grown at 37°C in competence medium in the presence of 0.5% xylose and in the absence or in the presence of 1 mM IPTG. Phase contrast and GFP images were taken 7 h after depletion (~12 generations). (B) Spores of strain PG87 were heat shocked and germinated as described in the presence of 0.5% xylose and in the absence or in the presence of 0.5% xylose. Phase contrast and GFP images were taken 4 h after the beginning of germination. (C,D) Localization of FtsL in FtsW depleted cells and germinating spores. (C) Strain PG79 (Pspac-ftsW; Pxyl-gfp-ftsL) was grown at 37°C in competence medium in the presence of 0.5% xylose and in the absence or in the presence of 1 mM IPTG. Phase contrast and GFP images were taken 7 h after depletion (~12 generations). (D) Spores of strain PG79 were heat shocked and germinated as described in the presence of 0.5% xylose and in the absence of 0.5% xylose. Phase contrast and GFP images were taken 4 h after the beginning of germination. Scale bar indicates 5 μm.