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. 2016 Nov 15;7:1808. doi: 10.3389/fmicb.2016.01808

Figure 4.

Figure 4

The localization of FtsW, PBP 2B, and FtsL are interdependent. (A,B) Localization of FtsW in PBP 2B depleted cells and germinating spores. (A) Strain PG92 (Pspac-pbpB; Pxyl-ftsL, ftsW-gfp) was grown at 37°C in competence medium in the presence of 0.5% xylose and in the absence or in the presence of 1 mM IPTG. Phase contrast and GFP images were taken 5 h after depletion (8–9 generations). (B) Spores of strain PG92 were heat shocked and germinated as described in the presence of 0.5% xylose and in the absence or in the presence of 0.5% xylose. Phase contrast and GFP images were taken 4 h after the beginning of germination. (C,D) Localization of FtsW in FtsL depleted cells. Strain PG92 was grown in competence medium at 37°C in the presence of 1 mM IPTG and in the absence or in the presence of 0.5% xylose. Phase contrast and GFP images were taken after 4 h (C) or 5 h (D) of depletion. Scale bar indicates 5 μm.