Table 1.
List of Botrytis cinerea isolates used for the development of the assay.
| Isolate code | sdhB mutationa | Year of isolation | Origin |
|---|---|---|---|
| BcSV10 | Wild-type | 2014 | strawberry |
| Bc33 | Wild-type | 2010 | strawberry |
| BcSv14 | H272R | 2014 | strawberry |
| BcTPR48 | H272R | 2012 | tomato |
| BcSv44 | H272Y | 2014 | strawberry |
| Bc30 | H272Y | 2011 | strawberry |
| BcE114 | H272L | 2011 | strawberry |
| Bc278 | H272L | 2009 | strawberry |
| BcE98 | N230I | 2011 | strawberry |
| BcTKp12 | N230I | 2011 | tomato |
| BcS22 | P225F | 2010 | strawberry |
| BcS24 | P225F | 2010 | strawberry |
| BcM100 | P225H | 2013 | Cherry rootstock |
| BcM182 | P225H | 2013 | Cherry rootstock |
| BcB1 | P225Lb | – | grape |
| BcB2 | P225Tb | – | Laboratory |
| induced mutation |
asdhB mutations were identified using a PIRA–PCR technique (Veloukas et al., 2011) and confirmed by direct sequencing of sdhB using the primer pair IpBcBeg-IpBcend (Leroux et al., 2010). bIsolates possessing the P225L and P225T mutations were kindly offered to us by Dr. Gerd Stammler (BASF, Limburgerhof, Germany).