Figure 15.

Western blot analysis of aging-related proteins in the olfactory bulbs and hippocampi of 5-month old WT and Fus1 KO mice. Proteins involved in energy homeostasis, antioxidant machinery, autophagy, apoptosis, and brain activities were analyzed in the olfactory bulbs and hippocampi of 5-month old WT and Fus1 KO mice by Western blot and are presented as a relative expression or phosphorylation index. The numbers to the right of each western blot panel represent the fold change in protein expression in the WT relative to the KO. All band intensity levels were normalized to a corresponding β-actin level. Phosphorylation index (P-index) is the ratio of phosphorylated protein intensity over total protein band intensity. Only changes that showed more than 30% difference between WT and KO samples were taken into consideration and shown as numbers in black as opposed to numbers in gray.