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. 2016 Nov 15;10:523. doi: 10.3389/fnins.2016.00523

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Copyright © 2016 Reyes-Aguirre and Lamas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of markers associated to DNA methylation and demethylation after retinal injury in vivo. (A) RT-PCR analysis for genes encoding for DNA methyltransferases (Dnmt1, Dnmt3a, and Dnmt3b), and a Gadd45 protein, associated with repair-based DNA demethylation. C, control; +, embryonic cDNA used as positive control. (B) qPCR analysis of Dnmt3b and Gadd45b. (C) qPCR for DNA demethylation-associated Tet proteins. (D) Comparison between expression changes in pluripotency-associated genes and methylation and demethylation-associated markers (ANOVA; ^***p < 0.001; ^**p < 0.01; ^*p < 0.05).