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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Nat Chem Biol. 2016 May 16;12(7):523–530. doi: 10.1038/nchembio.2084

Figure 1. Mechanism and synthesis of the activity based probe Ub-Dha.

Figure 1

In situ activation of Ub-Dha with E1 and ATP results in a mechanism based ABP for E1, E2 and Cys dependent E3 enzymes. Pathway a describes the covalent trapping of the enzyme (E-UbDha = thioether-linked adduct), while pathway b depicts the native transthioesterification processing of the probe (E~UbDha = thioester intermediate of conjugate) by the cascade.