FIG 6.

LOH analysis of chromosome 17q with double-strand break-induced thymidine kinase (TK) revertants from TSCER2 and BLM-TSCER2 cells. We analyzed frameshift mutations in exons 4 and 7 of the TK gene and seven microsatellite loci that are heteromorphic in these cells. We examined 40 TK revertants from TSCER2 cells (left) and 85 TK revertants from BLM-TSCER2 cells (right). Open squares indicate non-LOH. Solid red and blue squares indicate homo-LOH retaining an “a” allele and homo-LOH retaining a “b” allele, respectively. The LOH revertants were classified into crossover and noncrossover types.