TABLE 3.
Primers for qPCR analysis of zebrafish genes
| Zebrafish gene product | Primera | Sequence(5′–3′) |
|---|---|---|
| GAPDH | FP | GCTTCTCACAAACGAGGACACA |
| RP | GTCAAGAAAGCAGCACGGGT | |
| DHX33 | FP | TGCCGCTCTACGCCTCACT |
| RP | GCAATGTTGGTGGACAGAATCA | |
| Cyclin A2 | FP | GCGGGAAATGGAGGTCAAG |
| RP | TCCCACTTCCACCAACCAGT | |
| Cyclin B2 | FP | CACAAACCAGTTCAGACAAAGAAGG |
| RP | GCTGGACCTGGGCACTTTTG | |
| Cyclin D1 | FP | GAACACTTCCTTGCCAAACTGC |
| RP | GTCGGTGCTTTTCAGGTACAGTC | |
| Cyclin E2 | FP | CCCGCAACACATTACAAGAAAG |
| RP | TAAGACACTGGCACTAACATCACC | |
| E2F1 | FP | GCAACGCCACATCATACAAGC |
| RP | CGTAGCGGGATTTCTCCACA | |
| MCM4 | FP | GAACGCTGGTCTGGATCTCAA |
| RP | CGCTGTAGGGTCGCACTTG | |
| MCM6 | FP | ACGAAGGTTTGCGTGGGC |
| RP | CTGGTGCAGAGGTTATGGTAGAGG | |
| MCM7 | FP | GGTTGTGCGTGATGTGAAGGC |
| RP | ATGATGAGCGGCGTGAAGC | |
| CDC6 | FP | GTGTTCCTCAAGTGGCTCGG |
| RP | CTTCACACAGCTTGCCCAACT | |
| CDC20 | FP | GCCCTCTACTGCGCCAAATC |
| RP | ATAACCCTCTGGTGCGTAAAGC |
a
FP, forward primer; RP, reverse primer.