FIG 2.

Identification of Acr-modified histone lysines in cells exposed to Acr. (A) FDP-K IP was carried out to enrich Acr-modified histones from acid extracts of BEAS-2B cells. Coomassie blue (CB) staining and Western blot (WB) analysis of FDP-K IP sample demonstrate an ∼55-kDa band containing FDP-K, H3, and H4. The ∼55-kDa gel band was excised and digested with trypsin, and the resultant peptides were extracted and subjected to LC-MS/MS analysis to determine their composition and possible Acr-modified histone lysines. Lane M, molecular size markers. (B) The peptide corresponding to Acr-modified ₄GK(A)GGK(MP)GLGKGGAK(D)₁₆ (MH3⁺³ 419.60) from H4 identified by LC-MS/MS. A, aldimine; D, dimethyl.