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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Curr Biol. 2015 Dec 31;26(2):139–149. doi: 10.1016/j.cub.2015.11.053

Figure 4. COP1 is responsible for the 26S proteasome-mediated EBF1 and EBF2 protein degradation.

Figure 4

Western blots showing the EBF1(A, left panel) or EBF2 (B, left panel) protein levels. Seedlings over-expressing EBF1-TAP of EBF2-TAP in Col-0 (WT) and cop1-4 mutant backgrounds were grown on 1/2MS medium in the dark for 4 days without (DMSO) or with MG132 pre-treatment for 12 h before harvesting. Col-0 was used as a negative control. RPT5 was used as a loading control. Right panel shows the quantification analysis of the three biological replicates of EBF1 (A, right panel) or EBF2 (B, right panel) protein levels after normalizing to RPT5. The protein levels EBF1-TAP/EBF2-TAP in Col-0 backgrounds without MG132 treatment (DMSO) was set as 1. Mean ±s.d., n=3.