Table 1.
Effect of wild-type Human cTnI and mutants on the Ca2+ sensitivity of force development (pCa50) and the Hill coefficient (nH) in skinned porcine cardiac muscle fibers.
| Human cTnI Mutant | pCa50 | Difference from Wild-type (ΔpCa50) | Hill coefficient (nH) | Number of experiments (n) |
|---|---|---|---|---|
| Wild-type (204R) | 5.66 ± 0.07 | - | 1.85 ± 0.23 | 3 |
| 204H | 5.95 ± 0.08* | 0.28 | 1.52 ± 0.08* | 3 |
| 204G | 6.04 ± 0.17* | 0.37 | 1.51 ± 0.09* | 3 |
| 204C | 5.94 ± 0.07* | 0.27 | 1.35 ± 0.07* | 3 |
| 204W | 5.89 ± 0.03* | 0.22 | 1.50 ± 0.03* | 3 |
| 204Q | 5.98 ± 0.04* | 0.31 | 1.46 ± 0.02* | 3 |
| 204P | 6.01 ± 0.01* | 0.35 | 1.40 ± 0.06* | 3 |
The pCa50 and nH values are the average of 3 independent fiber experiments, and the errors are the standard deviation (S.D.) values.
*
Indicates that the pCa50 values and Hill coefficient for the respective TnI mutants are significantly different from wild-type HcTnI (p < 0.05).