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. 2016 Nov 15;6:37195. doi: 10.1038/srep37195

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Copyright © 2016, The Author(s)

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(A,B) Symptoms caused by wild type, the csrB and csrA mutants and their complementation strains on immature pear fruits. Immature pears were surface sterilized, pricked with a sterile needle and inoculated with 2 μL of bacterial suspensions. Symptoms were recorded and photos were taken at 4 and 7 days post-inoculation (dpi). (C) Growth of Erwinia amylovora wild type, mutants and complementation strains. Immature pears were surface sterilized, pricked with a sterile needle and inoculated with 2 μL of bacterial suspensions. Tissue surrounding the inoculation site was excised with a cork borer no.4 and homogenized in 1 mL of 0.5x PBS. Bacterial growth within the pear tissue was monitored after 1, 2 and 3 days post inoculation by dilution plating on LB with appropriate antibiotics.