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. 2016 Nov 15;6:37195. doi: 10.1038/srep37195

Figure 5. Relative expression of type III secretion genes and accumulation of HrpA protein.

Figure 5

Relative expression of T3SS genes in the grrA,csrB and csrA mutant strains compared to wild type in HMM (A) and in pears (B). Relative gene expression of selected genes was calculated by the 2−ΔΔCt method, utilizing the 16S rRNA as endogenous control and compared to wild type strain. Fold changes were the result of the mean of three replicates. Each experiment was performed at least two times with similar results. Error bars indicate standard deviations. (C) Accumulation of HrpA protein is controlled by CsrA. Abundance of HrpA-His6 protein in WT, the grrA, csrA, and csrB mutant strains was detected by western blot using anti-histidine protein antibody after grown in hrp-inducing minimal medium (HMM) at 18 °C for 6 h. Relative protein abundance was calculated using ImageJ software by utilizing the average pixel value of the signals and utilizing the WT sample as 100%. Cropped blot was displayed and full length blot was presented in Supplementary Fig. S4 as a presentative of results obtained in repeated experiments.