E. amylovora |
Ea1189 |
Wild type, isolated from apple |
71 |
Z2946∆flhD
|
flhD::Km; KmR-deletion mutant of flhD of Ea1189, KmR
|
14 |
∆csrA
|
csrA::Cm; CmR-deletion mutant of csrA of Ea1189, CmR
|
This study |
∆csrB
|
csrB::Cm; CmR-deletion mutant of csrB of Ea1189, CmR
|
13 |
∆glgCAP
|
CmR-deletion mutant of glgCAP operon (5.2 kb) of Ea1189, CmR
|
This study |
∆csrA/glgCAP
|
csrA::Cm, glgCAP::Km; KmR-deletion mutant of glgCAP into ∆csrA
|
This study |
Z2198∆grrA
|
grrA::Km; KmR-deletion mutant of grrA of Ea1189, KmR
|
14 |
DH10B E. coli strain
|
F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ |
Invitrogen, CA |
Plasmids |
|
|
pKD46 |
ApR, PBAD
gam bet exo pSC101 oriTS |
68 |
pKD32 |
CmR, FRT cat FRT tL3 oriR6Kγ bla rgnB
|
68 |
pkD13 |
KmR, FRT kan FRT tL3 oriR6Kγ bla rgnB
|
68 |
pHrpA-His6 |
803-bp DNA fragment containing promoter sequence of the hrpA gene and c-terminal His tag in pWSK29 |
8 |
pGem®T-easy |
ApR, PCR cloning vector |
Promega WI |
pCsrA |
A 824 bp fragment containing csrA gene in pGemT-easy vector |
This study |
pCsrB |
A 1.3-kb fragment containing csrB in pGemT-easy vector |
This study |