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. 2016 Nov 15;6:37195. doi: 10.1038/srep37195

Table 1. Bacterial strains and plasmids used in this study.

Strains or plasmids Description Reference or source
E. amylovora
Ea1189 Wild type, isolated from apple 71
Z2946∆flhD flhD::Km; KmR-deletion mutant of flhD of Ea1189, KmR 14
csrA csrA::Cm; CmR-deletion mutant of csrA of Ea1189, CmR This study
csrB csrB::Cm; CmR-deletion mutant of csrB of Ea1189, CmR 13
glgCAP CmR-deletion mutant of glgCAP operon (5.2 kb) of Ea1189, CmR This study
csrA/glgCAP csrA::Cm, glgCAP::Km; KmR-deletion mutant of glgCAP into ∆csrA This study
Z2198∆grrA grrA::Km; KmR-deletion mutant of grrA of Ea1189, KmR 14
DH10B E. coli strain F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ Invitrogen, CA
Plasmids    
pKD46 ApR, PBAD gam bet exo pSC101 oriTS 68
pKD32 CmR, FRT cat FRT tL3 oriR6Kγ bla rgnB 68
pkD13 KmR, FRT kan FRT tL3 oriR6Kγ bla rgnB 68
pHrpA-His6 803-bp DNA fragment containing promoter sequence of the hrpA gene and c-terminal His tag in pWSK29 8
pGem®T-easy ApR, PCR cloning vector Promega WI
pCsrA A 824 bp fragment containing csrA gene in pGemT-easy vector This study
pCsrB A 1.3-kb fragment containing csrB in pGemT-easy vector This study