Figure 2. Immunohistochemistry staining of laminin on BP scaffold and immunofluorescent staining of laminin (red) and eGFP (blue) on seeded BP scaffold (200×).

Laminin is present on the serous side of native BP, but absent on the fibrous side. ASB-14 scaffolds largely preserve laminin presence, whereas SDS scaffolds demonstrate disruption of laminin architecture. Following seeding, hMSC on the fibrous side of ASB-14 scaffolds are positive for laminin co-staining. No cells are evident on SDS scaffolds. Arrows indicates hMSC. Scale bar represents 100 μm.