Figure 4. Maximal seeding density and binding kinetics for hMSC on ASB-14 scaffolds.

(A) Adhesion of viable cells increases with cell seeding up to 60,000 cells/scaffold (***p < 0.005, n = 7 per group). Serous side supports a greater density of hMSC compared to the fibrous side (**p < 0.01). (B) No significant difference in kinetics of hMSC adhesion to BP ECM is found between fibrous and serous seeding (n = 4 per group/time point). Regardless of seeding location, number of adherent cells increases until 2 h of contact time (*p < 0.05). Data are shown as the mean ± standard deviation.