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. 2016 Nov 15;7:1702. doi: 10.3389/fpls.2016.01702

FIGURE 3.

FIGURE 3

Relative expression of cyclophilins in viruliferous and non-viruliferous whiteflies (NVBt) measured using quantitative RT-PCR (qRT-PCR), compared to the expression levels of the housekeeping gene β-actin gene. (A–C) Show relative expression of CypB, CypD, and CypG in viruliferous and NVBt, respectively, in Rickettsia-infected (R+) and uninfected (R-) whiteflies. Whiteflies were given acquisition access period for 10 h on Tomato yellow leaf curl virus (TYLCV)-infected plants, or healthy plants as control. (D) Relative expression of CypB (triangle columns), CypD (dotted columns), and CypG (vertical lines columns) in B. tabaci dissected midguts from viruliferous (MGR+TYLCV) and non-viruliferous (MGR+) Rickettsia-infected whitefly B adults after a 10 h acquisition access period on TYLCV-infected and healthy tomato (HT), respectively. Data shown are the means ± standard errors of the means of data from three independent experiments. Asterisk indicate statistically significant differences with P < 0.05 between viruliferous and non-viruliferous insects.