FIGURE 4.

Immunocapture-PCR assay to detect CypB-TYLCV CP interaction in viruliferous whiteflies (VBt). Lanes 1–8 and 10–17 represents microtiter plates coated with an anti-TYLCV CP and anti-CypB specific antibodies, respectively, and a PCR was performed on the caught virus-CypB complexes. Lanes 1, 2, 10, and 11 are leaf extract from HT, lanes 3, 4, 12, and 13 are leaf extract from TYLCV-infected tomato (TYT). Lanes 5, 6, 14, and 15 are extracts of NVBt, and lanes 7, 8, 16, and 17 are extracts of VBt. Lane 9 and 18 are genomic extracted from TYT (PCR positive control). TYLCV V61-C473 (Table 1) primers were used to amplify the virus bind to the anti-TYLCV CP and anti-CypB antibody.