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. 2016 Oct 12;35(22):2447–2467. doi: 10.15252/embj.201694574

Figure EV4. Chm7‐Snf7 interactions can occur outside of the NE.

Figure EV4

  1. Deconvolved inverted fluorescence micrographs of BiFC signal in wild‐type yeast strains expressing Chm7‐VN and the indicated VC fusions. Cell borders are outlined. Scale bar is 5 μm.
  2. Deconvolved inverted fluorescence micrographs of BiFC signal of Chm7‐VN and Snf7‐VC in the indicated null backgrounds. Cell borders are outlined. Scale bar is 5 μm.
  3. Deconvolved fluorescence micrographs of BiFC signal of Chm7‐VN and Snf7‐VC with NE/ER marker HDEL‐dsRED and merge. Note there is a small amount of bleed‐through of the HDEL‐dsRED into the Venus channel. Cell borders are outlined. Scale bar is 5 μm.
  4. Deconvolved fluorescence micrographs of Chm7‐GFP and Nup170‐mCherry (with merge) in the indicated strains. Scale bar is 5 μm.