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. 2016 Oct 12;35(22):2447–2467. doi: 10.15252/embj.201694574

Figure 6. CHM7 is required for SINC formation.

Figure 6

  1. Tenfold serial dilutions of the indicated yeast strains were grown on YPD at 23°C for 3 days before imaging.
  2. Schematic of Sna3‐mCherry localization. Sna3 accumulates in the vacuole (V) or class E compartment (E) in cells with, or lacking, ESCRT function, respectively.
  3. Deconvolved fluorescence micrographs of Sna3‐mCherry in the indicated genetic backgrounds. Scale bar is 5 μm.
  4. Deconvolved fluorescence micrographs (maximum intensity projections of a z‐series of images) of GFP‐Nup49 in the indicated yeast strains (arrows indicate SINCs). Scale bar is 5 ?m.
  5. Plot of proportion of cells in the indicated strains with SINCs. Error bars are the SD from the mean from three independent replicates quantifying > 300 cells for each strain. P‐values from unpaired Student's t‐test where ns is P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.