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. 2016 Oct 12;35(22):2447–2467. doi: 10.15252/embj.201694574

Figure 9. Chm7 protects nuclear compartmentalization.

Figure 9

  1. Deconvolved fluorescence micrographs of NLS‐GFP in the indicated yeast strains at either 30°C or after 2 h at 37°C. Scale bar is 5 μm.
  2. Plot of the mean nuclear to cytosolic fluorescence intensity ratio of NLS‐GFP from cells represented in (A). Error bars are SD from the mean from three independent replicates of 150 cells from each strain. Only chm7Δ and chm7Δapq12Δ cells have N:C ratios below 1.2 (dotted red line).
  3. Re‐plotting of data from (B) showing the percentage of cells with a N:C NLS‐GFP fluorescence ratio < 1.2. Error bars are SD from the mean from three independent replicates of 150 cells/strain. P‐values from Student's t‐test; *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001.
  4. Model of a proposed stepwise recruitment and activation of Chm7 and other ESCRTs at defective NPCs (bottom, red), or NE holes.