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. 2016 Oct 17;35(22):2484–2497. doi: 10.15252/embj.201694695

Figure 3. Formation of reduction‐resistant dimers is reversible and conserved between DNGR‐1 isoforms.

Figure 3

  1. 1 μg of purified long mouse DNGR‐1 ECD was transferred into 10 mM MES pH 6.1 and 0.5 μg into PBS. Half of the MES sample and the whole PBS sample were dialyzed against 2 l of PBS overnight at 4°C. After dialysis, all samples were prepared for reducing SDS–PAGE and Western blot.
  2. Supernatants after production of indicated murine proteins in 293F cells were harvested and diluted into indicated buffers at a 2:3 ratio. 1 μg of purified human DNGR‐1 ECD was diluted into the same buffers and all samples were analyzed using reducing SDS‐PAGE and Western blot.
Data information: HRP‐conjugated anti‐FLAG antibody was used for detection of all proteins. Numbers on the side of blots indicate positions of molecular weight markers. Reduction‐resistant dimers are indicated with an * and reduction‐sensitive protein with a +.Source data are available online for this figure.