Figure 1. The ~28-kb genomic island, GIVchS12, from Vibrio cholerae S12 containing a CRISPR-Cas module and type VI secretion auxiliary locus.

Schematic representation of GIVchS12 is shown in (A) with the VPI-1 att sites given as hatched boxes and the VPI-1 integrase as a black block arrow. Regions of nucleotide identity to VPI-1 and surrounding regions in V. cholerae N16961 are shown. All ORFs and their orientation are shown as block arrows. Numbers shown in, above, or below the block arrows correlate to the putative identification shown in (B). Primer binding sites are shown by black triangles with their direction of extension indicated by the direction of the triangle.