Table 1. Primers used in this study.

Primer	Sequence (5′-3′)	Target
ML126	ACTTCTCGAAAGCGGATCAA	attL end of GIVchS12
ML127	AAGCCATCACCATCGAAAAG	attR end of GIVchS12
ML130	GCTACCTTTGGCTTCAATCG	attR end of GIVchS12
ML131	TGGCAACAAGATGACTTTATCG	attL end of GIVchS12
ML134	TCCCTAGCTTCCGCTTGTAA	Between VC0848 and attR of GIVchS12
ML135	TCAGTGATGCAGGTTGTTCA	Within VC0816
ML136	GGGAATTTTGCAGTCTGAGG	Between VC0848 and attR of GIVchS12
ML137	ATAGGGAGTGGGGCGTTAAT	Within VC0816
Gap_F1	GCGTTTTTATCAATGGCAAACC	Within ORF 13 of GIVchS12
Gap_R1	ACACAGGGCTACCTCTAGATGG	Within and just past ORF 15 of GIVchS12