Table 1. SNV and INDEL Mutation Summary across Generations Passing All Filters.

	generation 16			generation 28			generation 50
	7564-F1	7564-F2	7564-F3	7564-F1	7564-F2	7564-F3	7564-F1	7564-F2	7564-F3
genome coverage (x)	65.15	102.48	70.1	81.34	63.85	79.76	111.06	106.81	86.12
% covered by ≥15 reads	94.6	96.7	94.9	95	86.6	89.6	97.5	97.5	96.6
SNVs Identified
total mutations	6	1	9	0	0	1	2	1	1
intergenic	5	0	5	0	0	0	1	0	0
intronic	1	1	4	0	0	0	0	0	0
synonymous	0	0	0	0	0	0	0	0	0
non-synonymous	0	0	0	0	0	1	1	1	1
genes mutated in all samples	none			none			Pf3D7_0321900: PfCARL
INDELs Identified
total mutations	1	2	2	0	0	0	1	1	2
intergenic	0	0	1	0	0	0	0	1	2
intronic	1	2	1	0	0	0	1	0	0
synonymous	0	0	0	0	0	0	0	0	0
non-synonymous	0	0	0	0	0	0	0	0	0
genes mutated in all samples	none			none			none