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. 2016 Nov 15;6:36926. doi: 10.1038/srep36926

Figure 2. LXRα modulates Angptl8 gene induction during refeeding.

Figure 2

(a) T0901317 increases Angptl8 accumulations in a time-dependent manner. Mouse primary hepatocytes were treated with T0901317 (10 nM) for the indicated time points, after which immunoblot assay was performed. (b,c) Cultured primary hepatocytes were infected with Ad-GFP or Ad- LXRα. The infected cells were cultured O/N in serum-free M199 medium and then treated with T0901317 (10 nM) for 4 hours. Angptl8 expression was analyzed by qPCR (b), and immunoblot assay (c), respectively. (d) Knockout of Lxrα decreases Angptl8 amounts in vivo. (e,f) Mice were randomly separated into four groups as indicated. For group 1, mice were fed ad libitum; for group 2, mice were fasted from Day1-ZT12 to Day2-ZT16; for two refed groups, mice were fasted from Day1-ZT12 to Day2-ZT12, followed by refed 4 h from Day2-ZT12 to Day2-ZT16. For the refed group, animals were intraperitoneally injected with vehicle (Veh) and GSK 2033 (20 mg/Kg) at Day2-ZT12, respectively. All animals were euthanized at Day2-ZT16. Liver and plasma samples were harvested. Temporal expression of Angptl8 was analyzed by qPCR (e), and immunoblot assay (f), respectively. Data are represented as mean ± s.e.m, n = 3–5, *p < 0.05, **p < 0.01.