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. 2016 Nov 15;6:37033. doi: 10.1038/srep37033

Figure 1. An increase in GluN2C expression, but not GluN2A or GluN2B, was observed following ischemia.

Figure 1

(a) Summary data showing quantitative real time PCR results of GluN2A, GluN2B and GluN2C mRNA levels extracted from acute hippocampal slices following 4 min OGD and 90 min reperfusion. Ct-values are normalized to β-actin internal control. (b,c) Representative Western blot showing GluN2C upregulation in membrane fraction of acute hippocampal slices (labeled Hp in figure) following 4 min OGD and 3 hr reperfusion (b) or 12 min GCI and 6 hr reperfusion (c) (full-length blots are presented in Supplementary Fig. S8). Cerebellum lysate (labeled Cb in figure) was used as positive control for GluN2C expression. Graphs depict mean ± SEM (n = 3, *p < 0.05, Student’s t-test).