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. 2016 Nov 15;6:37033. doi: 10.1038/srep37033

Figure 2. GluN2C−/− mice display less CA1 surviving neurons and hippocampal-dependent functional loss following global cerebral ischemia (GCI).

Figure 2

Both WT and KO (except sham groups) mice were induced with 15 min GCI. (a) Representative images of hippocampal sections comparing neuronal survival of WT sham, KO sham, WT GCI and KO GCI groups. Sections were stained with NeuN antibody (green). The left column depicts the hippocampus imaged using 10× objective and the right column images represent magnified CA1 areas (using 40× objective) highlighted by the white box. Cells that stained positively for NeuN staining were identified as surviving neurons. Scale bar, 50 μm. (b) Quantitiative summary of data showing the average number of NeuN+ cells (surviving neurons) per 320 μm length of medial CA1 region. (c) Average alternation percentage defined as “the number of alternating triads/(the total number of arm entries –2)” from Y-maze test at ischemia/reperfusion day 3 and 7 (n = 8 mice per GCI group, 3 mice per sham group, *p < 0.05, One-way ANOVA and Bonferroni test).