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. 2016 Nov 15;6:37019. doi: 10.1038/srep37019

Figure 6. EnSCs inhibited AKT phosphorylation in both paracrine way and direct cell-cell contact way.

Figure 6

(A,B) The effects of EnSCs on the levels of phospho-AKT (Ser473) and PTEN in the xenografted tumor tissues were tested by IHC using antibodies against phospho-AKT (Ser473) and PTEN (n = 5; Scale bar = 100 μm). H-score system was used for semi-quantification between two groups. (C) Diagrammatic drawing of three methods to investigate the inter-communication between EnSCs and EOC cells, including using EnSC-CM (a), transwell system (b) and direct mixed co-culture of EnSCs with EOC cells (c). (D) Real-time PCR was used to test the effects of EnSCs on the transcription of AKT genes (AKT1, AKT2, and AKT3) and PTEN in EOC cells cultured with EnSCs in transwell system for 48 hours (n = 3; performed in triplicate). (E,F) Western blot was performed to test the effects of EnSCs on the levels of total-AKT, phospho-AKT (Ser473) and PTEN in EOC cells in different culture methods. The amount of protein loaded was normalized against GAPDH (n = 3; performed in triplicate). Ordinary one-way ANOVA was used for statistic analysis. All data were shown as means ± SEM. ns, no statistical significance; *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001.