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. 2016 Nov 15;6:37019. doi: 10.1038/srep37019

Figure 7. EnSCs promoted nuclear translocation of FoxO3a and inhibited the pro-angiogenetic ability of EOC cells in vivo.

Figure 7

(A) The effects of EnSCs on the levels of FoxO3a in tumor tissues were tested by IHC staining. Positively-stained cell ratios were measured and results were shown as averages of five randomly selected fields ± SEM (n = 5; Scale bar = 100 μm). (B) Western blot was performed to test the effects of EnSCs on FoxO3a levels within EOC cells in different culture methods. The amount of protein loaded was normalized against GAPDH (n = 3; performed in triplicate). Ordinary one-way ANOVA was used for statistic analysis. (C,D) The effects of EnSCs on the pro-angiogenetic ability of EOC cells in vivo were tested by IHC analysis using antibodies against VEGFA and HIF-1α. H-score system was used for semi-quantification between two groups (n = 5; Scale bar = 100 μm). All data were shown as means ± SEM. *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001.