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. 2016 Nov 14;9:577. doi: 10.1186/s13071-016-1862-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Indirect immunofluorescence assay for the recognition of native MSA-2a₁, MSA-2b and MSA-2c. Smears of B. bovis-infected erythrocytes (CC) were incubated with pools of sera from mice immunized with each recombinant protein (1:100 dilutions) or control sera (1:50 dilution). Reactions were detected with FITC-conjugated anti-mouse IgG antibodies (FITC). Nuclei were detected by DAPI staining (DAPI). Slides were observed with 400× magnification. Scale-bars: 10 μm