Table 1.

Phosphoprotein mutant set

Standard Name	Systematic name	Position of mutation within protein	Mutation type	Mutant nomenclature	Protein type	Pathways contribution	Evidence type
Crz1	YNL027W	409, 410, 423, 427, 429	p-deletion	Crz1 OUT	TF	HOG pathway	ch [38, 61]
Cys3	YAL012W	39, 40	p-deletion	Cys3 OUT	Enzyme	metabolic enzyme	dif [11, 30]
Gln3	YER040W	469, 471, 473	p-deletion	Gln3 site 1 OUT	TF	TOR signaling	dif [4, 11]
Gln3	YER040W	469	p-deletion	Gln3 site 2 OUT	TF	TOR signaling	dif [4, 11]
Gpd1	YDL022W	24, 27	p-deletion	Gpd1 OUT	Enzyme	metabolic enzyme	ch [11, 20]
Gpd2	YOL059W	72, 75	p-deletion	Gpd2 OUT	Enzyme	metabolic enzyme	ch [20]
Gut1	YHL032C	96	p-deletion	Gut1 OUT	Enzyme	metabolic enzyme	dif [11]
Hog1	YLR113W	174, 176	p-deletion	Hog1 OUT	Kinase	HOG pathway	ch [31, 62]
Lys20	YDL182W	395, 396	p-deletion	Lys20 OUT	Enzyme	metabolic enzyme	ch [4, 11, 26]
Nbp2	YDR162C	194, 196	p-deletion	Nbp2 OUT	Adapter	HOG pathway	dif [4, 30]
Nbp2	YDR162C	194, 196	p-mimic	Nbp2 IN	Adapter	HOG pathway	dif [4, 30]
Pbs2	YJL128C	508	p-deletion	Pbs2 OUT	Kinase	HOG pathway	ch [15]
Pbs2	YJL128C	508	p-mimic	Pbs2 IN	Kinase	HOG pathway	ch [15]
Pda1	YER178W	313	p-deletion	Pda1 OUT	Enzyme	metabolic enzyme	ch [63]
Pda1	YER178W	313	p-mimic	Pda1 IN	Enzyme	metabolic enzyme	ch [63]
Pfk1	YGR240C	895	p-deletion	Pfk1 OUT	Enzyme	metabolic enzyme	dif [4, 11]
Pfk2	YMR205C	163	p-deletion	Pfk2 OUT	Enzyme	metabolic enzyme	difa [4, 11]
Pfk2	YMR205C	163	p-mimic	Pfk2 IN	Enzyme	metabolic enzyme	difa [4, 11]
Put3	YKL015W	788	p-deletion	Put3 OUT	TF	TOR signaling	ch [18]
Rim15	YFL033C	1061	p-deletion	Rim15 OUT	Kinase	TOR signaling	ch [64]
Rim15	YFL033C	1061	p-mimic	Rim15 IN	Kinase	TOR signaling	ch [64]
Sko1	YNL167C	108, 113, 126	p-deletion	Sko1 OUT	TF	HOG pathway	ch [65]
Smp1	YBR182C	348, 357, 365, 376	p-deletion	Smp1 OUT	TF	HOG pathway	ch [66]
Ste50	YCL032W	202	p-deletion	Ste50 OUT	Adapter	HOG pathway	ch [37]
Str3	YGL184C	456	p-deletion	Str3 OUT	Enzyme	metabolic enzyme	htp [30]
Tco89	YPL180W	287, 288, 290	p-deletion	Tco89 OUT	Regulator	TOR signaling	dif [4]
Thr4	YCR053W	408	p-deletion	Thr4 OUT	Enzyme	metabolic enzyme	dif [11]
Tip41	YPR040W	79	p-deletion	Tip41 OUT	Regulator	TOR signaling	dif [4]
Tkl1	YPR074C	335	p-deletion	Tkl1 OUT	Enzyme	metabolic enzyme	htp [30]
Tkl1	YPR074C	335	p-mimic	Tkl1 IN	Enzyme	metabolic enzyme	htp [30]
Yap4	YOR028C	192, 196	p-deletion	Yap4 OUT	TF	HOG pathway	ch [19]
Zwf1	YNL241C	183, 188	p-deletion	Zwf1 OUT	Enzyme	metabolic enzyme	dif [11]

TF transcription factor, ch previously characterized phosphosite, dif phosphosite shown in previous phosphoproteomics studies to change in degree of phosphorylation in different conditions, htp phosphosite detected in high throughput phosphoproteomics studies, no data on differential phosphorylation

^aThe previously shown functionality of phosphorylation of Pfk2 S163 is potentially a result of increased expression from the plasmid used in that study (Additional file 2: Figure S6)