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. 2016 Nov 15;15:195. doi: 10.1186/s12934-016-0594-4

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — CRISPRi process used in this study. The crRNA was expressed on a plasmid and Cascade was activated for expression with the constitutive promoter J23119. Cascade mediates the maturation of crRNA and forms complex with the crRNA. The complex then binds to a target site to disturb transcription. RNAP indicates RNA polymerase, PAM indicates protospacer adjacent motif