Fig. 2.

Verification of endogenous CRISPRi using GFP. a Spacers designed to target gfp on the plasmid. The red circles indicate the PAM sequence; red lines indicate the spacer. b Endogenous CRISPRi repressed gfp expression. The strains were cultured in a 96-well plate in 200 μL LB medium, with or without 0.2% l-arabinose. The error bars indicates the standard deviations of eight biological replicates. The 0 indicates the control and T1, T2, NT1, and NT2 indicate the corresponding spacer names