Table 2.
Growth parameters from S. cerevisiae strains cultured with sucrose with or without 2-deoxy-d-glucose
| Strain | µsuc (h−1) | µsuc-2DG (h−1) | µsuc/µ suc-2DG | ODmax suc | ODmax suc-2DG | ODmax suc/ODmax suc-2DG |
|---|---|---|---|---|---|---|
| S288c | 0.165 ± 0.002 | 0.074 ± 0.002** | 2.23 | 7.2 ± 0.2 | 2.4 ± 0.1** | 3.0 |
| Sccyc8 − + YEpNP-CYC8 | 0.105 ± 0.003 | 0.082 ± 0.003** | 1.28 | 5.2 ± 0.2 | 3.73 ± 0.03** | 1.39 |
| Sc cyc8− + YEpNP | 0.068 ± 0.001 | 0.072 ± 0.001 | 0.94 | 2.7 ± 0.2 | 2.5 ± 0.3 | 1.08 |
| Sctup1 − + YEpNP-TUP1 | 0.095 ± 0.001 | 0.079 ± 0.002** | 1.20 | 4.0 ± 0.2 | 3.16 ± 0.06** | 1.27 |
| Sc tup1− + YEpNP | 0.077 ± 0.005 | 0.069 ± 0.001 | 1.10 | 3.57 ± 0.07 | 3.5 ± 0.2 | 1.02 |
Strains were cultured at 22 °C in SD medium supplemented only with 2% sucrose (suc) or that in addition to 0.02% 2-deoxy-d-glucose (suc-2DG). Each µ value corresponds to the average of three independents cultures and errors to the standard deviation. ODmax: maximum value of OD600 reached during the time period studied
** p < 0.01, Student’s t test (compared to the control condition)