Table 2.
List of conserved miRNAs and their recoveries profiling in response to CBCVd-infection in Humulus lupulus
| Family | miRNA name | Reference miRNA | Sequence (5′-3′) | Length | Normalized value | (log2 IS/HS) | P-value | Significance lable | |
|---|---|---|---|---|---|---|---|---|---|
| Healthy sample (HS) | Infected sample (IS) | ||||||||
| MIR156 | hop-miR156 | csi-miR156 | UGACAGAAGAGAGUGAGCAC | 20 | 9.926687278 | 46.07808544 | 2.214696525 | 1.47376E-85 | ** |
| MIR159 | hop-miR159a | ath-miR159a | UUUGGAUUGAAGGGAGCUCUA | 21 | 330.8895759 | 3017.517405 | 3.188940344 | 0 | ** |
| MIR159 | hop-miR159c | sof-miR159c | CUUGGAUUGAAGGGAGCUCC | 20 | 166.8234945 | 970.7829107 | 2.540826227 | 0 | ** |
| MIR159 | hop-miR319a | ath-miR319a | UUGGACUGAAGGGAGCUCCC | 20 | 165.5826586 | 242.9628926 | 0.553184401 | 3.286E-125 | |
| MIR160 | hop-miR160a-5p | ath-miR160a-5p | UGCCUGGCUCCCUGUAUGCCA | 21 | 7.445015459 | 37.08877273 | 2.31663578 | 8.88414E-72 | ** |
| MIR160 | hop-miR160f-5p | osa-miR160f-5p | UGCCUGGCUCCCUGAAUGCCA | 21 | 1.102965253 | 8.926450386 | 3.016699258 | 4.40629E-22 | ** |
| MIR162 | hop-miR162a-5p | aly-miR162a-5p | GGAGGCAGCGGUUCAUCGAUC | 21 | 23.43801163 | 38.03170763 | 0.698352534 | 2.60746E-25 | |
| MIR164 | hop-miR164a | ath-miR164a | UGGAGAAGCAGGGCACGUGCA | 21 | 0.41361197 | 9.115037366 | 4.461898728 | 9.32838E-28 | ** |
| MIR164 | hop-miR164g-3p | zma-miR164g-3p | CACGCGCUCCCCUUCUCCACC | 21 | 0.275741313 | 7.229167566 | 4.712442189 | 1.06026E-22 | ** |
| MIR166 | hop-miR166a | pvu-miR166a | UCGGACCAGGCUUCAUUCCCC | 21 | 23356.25433 | 12308.50642 | −0.924153218 | 0 | |
| MIR166 | hop-miR166e-5p | aly-miR166e-5p | UGAAUGUUGUCUGGCACGAGG | 21 | 23619.86303 | 12462.14195 | −0.922448543 | 0 | |
| MIR166 | hop-miR166m-5p | zma-miR166m-5p | GGAAUGUUGGCUGGCUCGAGG | 21 | 23621.93109 | 12466.91949 | −0.922021883 | 0 | |
| MIR167 | hop-miR167-3p | ahy-miR167-3p | GAUCAUGUGGCAGUUUCAUC | 20 | 2.48167182 | 159.4188604 | 6.005366167 | 0 | ** |
| MIR167 | hop-miR167a-5p | ath-miR167a-5p | UGAAGCUGCCAGCAUGAUCUA | 21 | 3.998249043 | 184.3752041 | 5.527132499 | 0 | ** |
| MIR167 | hop-miR167c-5p | tae-miR167c-5p | UGAAGCUGCCAGCAUGAUCUGC | 22 | 2.757413133 | 163.0648754 | 5.885986808 | 0 | ** |
| MIR168 | hop-miR168a-5p | ath-miR168a-5p | UCGCUUGGUGCAGGUCGGGAA | 21 | 186.8147398 | 189.0898786 | 0.017463854 | 4.97322E-31 | |
| MIR169 | hop-miR169b-5p | ath-miR169b-5p | CAGCCAAGGAUGACUUGCCGGC | 22 | 3.308895759 | 4.90326148 | 0.567391856 | 0.00076095 | |
| MIR169 | hop-miR169c | ath-miR169c | CAAGGAUGACUUGCCGGCGAC | 21 | 7.858627429 | 4.274638213 | −0.878475035 | 0.160709021 | |
| MIR169 | hop-miR169h | ath-miR169h | UAGCCAAGGAUGACUUGCCUG | 21 | 1.24083591 | 2.388768413 | 0.94495465 | 0.003574489 | |
| MIR169 | hop-miR1691-3p | gma-miR169l-3p | CGCUGGCAAGUUGUCUUUGGC | 21 | 8.134368742 | 4.52608752 | −0.84576591 | 0.191601254 | |
| MIR171 | hop-miR171 | ctr-miR171 | UUGAGCCGCGUCAAUAUCUCC | 21 | 0.689353283 | 8.549276426 | 3.632486885 | 1.14049E-23 | ** |
| MIR171 | hop-miR171b | osa-miR171b | UGAUUGAGCCGUGCCAAUAUC | 21 | 1.102965253 | 28.60235863 | 4.696674873 | 1.79944E-86 | ** |
| MIR171 | hop-miR171f | vvi-miR171f | UUGAGCCGCGCCAAUAUCACU | 21 | 1.930189193 | 1.571558167 | −0.296546594 | 0.681310925 | |
| MIR171 | hop-miR171f-5p | zma-miR171f-5p | CGAUGUUGGCAAGGUUCAAUC | 21 | 0.551482627 | 3.143116333 | 2.510808328 | 1.38415E-07 | ** |
| MIR172 | hop-miR172c | ath-miR172c | AGAAUCUUGAUGAUGCUGCAG | 21 | 0.82722394 | 2.26304376 | 1.451914639 | 0.000504687 | |
| MIR172 | hop-miR172e-5p | stu-miR172e-5p | GCAUCAUCAUCAAGAUUCACA | 21 | 3.860378386 | 7.857790833 | 1.025381501 | 2.04099E-08 | |
| MIR390 | hop-miR390a-3p | aly-miR390a-3p | CGCUAUCUAUCCUGAGUUUCA | 21 | 13.64919501 | 94.29349 | 2.788342304 | 4.8357E-208 | ** |
| MIR390 | hop-miR390a-5p | ath-miR390a-5p | AAGCUCAGGAGGGAUAGCGCC | 21 | 12.4083591 | 94.23062767 | 2.92488371 | 2.9794E-215 | ** |
| MIR395 | hop-miR395a | sly-miR395a | UGAAGUGUUUGGGGGAGCUCC | 21 | 7.169274145 | 97.81378029 | 3.770138765 | 9.4472E-263 | ** |
| MIR395 | hop-miR395d | ath-miR395d | CUGAAGUGUUUGGGGGAACUC | 21 | 5.514826266 | 40.10616441 | 2.862436657 | 3.88981E-91 | ** |
| MIR395 | hop-miR395e-5p | aly-miR395e-5p | AGCUCCUCUGAAGACUUCAGU | 21 | 7.031403489 | 95.99077282 | 3.771011143 | 6.4322E-258 | ** |
| MIR395 | hop-miR395g-5p | aly-miR395g-5p | AAUUCCUCUGAAGACUUCACU | 21 | 18.10435 | 3.860378 | 2.229522 | 1.01823E-34 | ** |
| MIR396 | hop-miR396b-5p | ath-miR396b-5p | UUCCACAGCUUUCUUGAACUU | 21 | 21.64569309 | 80.52664046 | 1.89538615 | 1.4111E-129 | |
| MIR398 | hop-miR398b-5p | zma-miR398b-5p | GGGGUUGCCUGAGAACACAUG | 21 | 160.619315 | 290.9897101 | 0.857322746 | 1.2737E-219 | |
| MIR399 | hop-miR399b | ath-miR399b | UGCCAAAGGAGAGUUGCCCU | 20 | 0.01 | 115.1637824 | 13.49139946 | 0 | ** |
| MIR399 | hop-miR399b-5p | aly-miR399b-5p | GGGCGUCUCUCCCUUGGCACG | 21 | 3.584637073 | 127.2962115 | 5.150218613 | 0 | ** |
| MIR399 | hop-miR399c-3p | ath-miR399c-3p | UGCCAAGAGGAGUUGCCCUGU | 21 | 65.48856191 | 305.3851829 | 2.22131521 | 0 | ** |
| MIR399 | hop-miR399d | ath-miR399d | UGCCAAAGGAGAUUUGCCCCG | 21 | 0.01 | 2.07445678 | 7.69658979 | 7.5833E-08 | ** |
| MIR399 | hop-miR399e | ptc-miR399e | CGCCAAAGGAGAGUUGCCCU | 20 | 1.24083591 | 117.0496522 | 6.559664494 | 0 | ** |
| MIR399 | hop-miR399g-5p | zma-miR399g-5p | GGGGCAUCCACUCUUUGGCAAG | 22 | 68.10810438 | 322.8609097 | 2.245014394 | 0 | ** |
| MIR403 | hop-miR403-3p | ath-miR403-3p | UUAGAUUCACGCACAAACUCG | 21 | 1.378706566 | 140.7487494 | 6.673662856 | 0 | ** |
| MIR408 | hop-miR408-3p | ath-miR408-3p | AUGCACUGCCUCUUCCCUGGC | 21 | 6.342050206 | 22.6304376 | 1.835243278 | 1.574E-36 | |
| MIR477 | hop-miR473a-3p | ptc-miR473a-3p | GGAGCCUUAAGGGGAGAGUGG | 21 | 243.4795796 | 330.9072876 | 0.442626284 | 1.4165E-142 | |
| MIR477 | hop-miR477a | nta-miR477a | ACUCUCCCUCAAGGGCUUCU | 20 | 3.860378386 | 2.954529353 | −0.385813932 | 0.726562864 | |
| MIR482 | hop-miR482a | ghr-miR482a | UCUUUCCUACUCCACCCAUACC | 22 | 241.6872611 | 1445.142028 | 2.579997949 | 0 | ** |
| MIR482 | hop-miR482b-3p | gma-miR482b-3p | UUUUCCCAACACCUCCCAUACC | 22 | 803.2344456 | 2464.203205 | 1.617228186 | 0 | |
| MIR482 | hop-miR482b-5p | gma-miR482b-5p | UAUGGGGGGAUUGGGCAAAGC | 21 | 803.2344456 | 2464.203205 | 1.617228186 | 0 | |
| MIR482 | hop-miR1448 | ptc-miR1448 | UACAUCCAACGUCUCCCACUGGG | 23 | 0.01 | 0.817210247 | 6.352635388 | 0.001758949 | ** |
| MIR529 | hop-miR529b | osa-miR529b | AGAAGAGAGAGAGUACAGCUU | 21 | 2.48167182 | 2.325906087 | −0.093519498 | 0.322219956 | |
| MIR535 | hop-miR535a | ppt-miR535a | UGACAACGAGAGAGAGCACGC | 21 | 17.78531471 | 47.02102034 | 1.402619343 | 4.26882E-58 | |
| MIR827 | hop-miR827a | ghr-miR827a | UUAGAUGACCAUCAACAAACA | 21 | 4.411861013 | 173.18571 | 5.294788742 | 0 | ** |
| MIR828 | hop-miR828-3p | aly-miR828-3p | AGAUACUCAUUUGAACAAGAAA | 22 | 4.549731669 | 9.177899693 | 1.012382578 | 1.61686E-09 | |
| MIR1528 | hop-miR1528 | gma-miR1528 | AAAAUAGAUCAAAAUAGUACUCU | 23 | 0.82722394 | 2.07445678 | 1.326383757 | 0.001469896 | |
| MIR2673 | hop-miR2673a | mtr-miR2673a | CCUCUUCGUCUUCAUCUUCGG | 21 | 0.689353283 | 2.388768413 | 1.792951557 | 7.85388E-05 | |
| MIR3441 | hop-miR3441-3p.1 | aly-miR3441-3p.1 | UGUAAUAAUGAUGUUUUGGGGUA | 23 | 0.01 | 0.691485593 | 6.111627289 | 0.004806101 | ** |
| MIR4402 | hop-miR4402 | gma-miR4402 | UUGAAUAUUAUGUGCCCCAGAC | 22 | 0.137870657 | 0.817210247 | 2.567391856 | 0.011486802 | ** |
| MIR5255 | hop-miR5225-3p | ppe-miR5225-3p | UCUCCCCGCGACUGAAGCCCC | 21 | 16.68234945 | 20.55598082 | 0.301235726 | 3.28085E-08 | |
| MIR6297 | hop-miR6297a | ppe-miR6297a | AAUAAUUUAUGGUGUCGAAAACU | 23 | 0.01 | 0.56576094 | 5.822120672 | 0.013132046 | ** |
| MIR7494 | hop-miR7494 | ghr-miR7494 | CGUAUCAGGGAGCUUGUGGACU | 22 | 2.343801163 | 7.669203853 | 1.710226635 | 1.55117E-12 | |
| MIR7755 | hop-miR7755-3p | bdi-miR7755-3p | AAUAAUUUACGAUGUCGAAAACA | 23 | 0.551482627 | 0.56576094 | 0.03687714 | 0.584473414 | |
| MIR7755 | hop-miR7755-5p | bdi-miR7755-5p | AUAAUUUACAGUGUCGAAAAUA | 22 | 0.01 | 0.75434792 | 6.237158171 | 0.002907523 | ** |
| MIR7815 | hop-miR7815 | ptc-miR7815 | UCUUAAAAUAAUUGGUGGGAC | 21 | 0.41361197 | 2.07445678 | 2.326383757 | 4.15138E-05 | ** |
| MIR7987 | hop-miR7987 | stu-miR7987 | UGACAAUUUGAUCAUAUUGACA | 22 | 0.01 | 1.32010886 | 7.044513093 | 3.1557E-05 | ** |
| MIR8562 | hop-miR8562a | atr-miR8562a | UCGGAAUUUUCUGAAAAUUUGCA | 23 | 1.516577223 | 1.257246533 | −0.270551386 | 0.6884511 | |
| MIR8693 | hop-miR8693 | gra-miR8693 | AUGAAAAUCUUGAUUUUGAAGGC | 23 | 0.137870657 | 2.137319107 | 3.954414979 | 6.80172E-07 | ** |
| MIR8712 | hop-miR8712 | gra-miR8712 | ACCUAUGUGGUGAUGCAUCGCCU | 23 | 0.41361197 | 0.75434792 | 0.866952138 | 0.140681352 | |
| MIR8744 | hop-miR8774 | gra-miR8774 | GAUUUUGAUUCAGAUAUGGAU | 21 | 1.24083591 | 4.14891356 | 1.741421256 | 1.97494E-07 | |