Table 3.
List of novel miRNAs and their recoveries profiling in response to CBCVd-infection in Humulus lupulus
| miRNA name | Sequence (5′-3′) | Length | Normalized value | Fold-change (log2 IS/HS) | P-value | Significance lable | |
|---|---|---|---|---|---|---|---|
| Healthy sample (HS) | Infected sample (IS) | ||||||
| hop-miR01 | UCUGAAUAAAUUAUGGUACCGAA | 23 | 3.033154446 | 3.20597866 | 0.079945862 | 0.105727 | |
| hop-miR02 | UUUCUGUUCGACUGGUAGAGA | 21 | 4.273990356 | 66.57120394 | 3.961242702 | 3.32E-184 | ** |
| hop-miR03 | UUGGUACCCUAAACUCAGAUU | 21 | 18.47466799 | 8.046377813 | −1.199137052 | 0.000135 | |
| hop-miR04 | UUUUUGGCACUGUAAAUUAUUCA | 23 | 0.01 | 2.26304376 | 7.822120672 | 1.68E-08 | ** |
| hop-miR05 | CCGAAACAUGGUCUAAACUGU | 21 | 0.01 | 1.571558167 | 7.29605186 | 4.23E-06 | ** |
| hop-miR06 | GUAAUUGACUGUUUGAACUCU | 21 | 0.01 | 0.817210247 | 6.352635388 | 0.001759 | ** |
| hop-miR07 | CUAAUGUCUCUGGCCGCGGCC | 21 | 0.275741313 | 0.56576094 | 1.03687714 | 0.177719 | |
| hop-miR08 | CUAGUUUUCGGUACUGUAAACU | 22 | 0.551482627 | 23.44764785 | 5.409983958 | 9.65E-75 | ** |
| hop-miR09 | GGGUACCAUAUGAGCAUUAUUGA | 23 | 1.792318536 | 5.154710786 | 1.524064425 | 4.58E-08 | |
| hop-miR10 | UAUAAAAUAGUUCAAAUAGACU | 22 | 0.01 | 0.56576094 | 5.822120672 | 0.013132 | ** |
| hop-miR11 | UCGGUGAACUCUCUUAUUCGC | 21 | 0.01 | 2.26304376 | 7.822120672 | 1.68E-08 | ** |
| hop-miR12 | AAUUAAAUUUGAGUUUAGGGUAC | 23 | 0.551482627 | 3.708877273 | 2.749595188 | 2.75E-09 | ** |
| hop-miR13 | GCUCUAAAUAACUACAAUAUACA | 23 | 0.137870657 | 3.39456564 | 4.62183964 | 4.50E-11 | ** |
| hop-miR14 | UUGGUACCCUAAACUCAGAUU | 21 | 19.02615062 | 2.388768413 | −2.993644805 | 7.35E-26 | ** |
| hop-miR15 | UUUAGAUCUUCCUAUAUAAUAUG | 23 | 0.01 | 1.005797227 | 6.65219567 | 0.000389 | ** |
| hop-miR16 | AAACGUGUAGAUCUUGAAAAAA | 22 | 0.137870657 | 2.137319107 | 3.954414979 | 6.80E-07 | ** |
| hop-miR17 | CUCUCGAAAUACUUGUUCUACC | 22 | 0.275741313 | 1.823007473 | 2.724933133 | 4.84E-05 | ** |
| hop-miR18 | GAAAACAGAUUAUUUGAACUC | 21 | 0.137870657 | 2.07445678 | 3.911346258 | 1.09E-06 | ** |
| hop-miR19 | UAAACUAUUCGAAAUUUUCUGAA | 23 | 0.82722394 | 1.382971187 | 0.741421256 | 0.052831 | |
| hop-miR20 | ACAUAUUUGGUACCAUAAACUC | 22 | 0.137870657 | 2.514493067 | 4.188880233 | 3.87E-08 | ** |
| hop-miR21 | UGAAUAGUUUACAGUACCGAAA | 22 | 0.01 | 3.96032658 | 8.629475594 | 2.15E-14 | ** |
| hop-miR22 | AGGAUAAAUCUAUGUUGCGGGUG | 23 | 0.01 | 0.628623267 | 5.974123765 | 0.007944 | ** |
| hop-miR23 | GUUUAAAUUUUAAGUCUUGUGGU | 23 | 0.01 | 0.75434792 | 6.237158171 | 0.002908 | ** |
| hop-miR24 | CACGGGUCGAGAACACUGAGAG | 22 | 22.886529 | 31.1168517 | 0.443197422 | 7.12E-15 | |
| hop-miR25 | UUGGAUUCAGGGACUAUUUUU | 21 | 0.689353283 | 8.737863406 | 3.663965116 | 2.80E-24 | ** |
| hop-miR26 | UCCGAAUAGUUUACAGUACCGAA | 23 | 0.01 | 1.194384207 | 6.900123184 | 8.62E-05 | ** |
| hop-miR27 | UCGACGCUGAACCAAAUAAACAU | 23 | 0.01 | 1.005797227 | 6.65219567 | 0.000389 | ** |
| hop-miR28 | GAUUGGUCAAGUGGCUUACAGG | 22 | 0.137870657 | 0.37717396 | 1.451914639 | 0.223324 | |
| hop-miR29 | AUCUGUAGCCAGACACAAAGG | 21 | 0.01 | 0.37717396 | 5.237158171 | 0.059312 | ** |
| hop-miR30 | UUUUUGGCACUGUAAAUUAUU | 21 | 0.41361197 | 1.382971187 | 1.741421256 | 0.003722 | |
| hop-miR31 | GCAAAUUUUCAGAAAAUUCUG | 21 | 0.689353283 | 2.64021772 | 1.937341466 | 1.63E-05 | |
| hop-miR32 | AGCCGAAAACAAAGUUCAAACUG | 23 | 0.137870657 | 1.760145147 | 3.67430706 | 1.17E-05 | ** |
| hop-miR33 | UGUGUAUAUUGUAGUUAUUUAGA | 23 | 0.137870657 | 0.9429349 | 2.773842734 | 0.004713 | ** |
| hop-miR34 | GUAAUUGACUGUUUGAACUCU | 21 | 0.01 | 1.194384207 | 6.900123184 | 8.62E-05 | ** |
| hop-miR35 | UUAAAAUAGAACCCUAAACCCGA | 23 | 0.965094597 | 7.5434792 | 2.966487812 | 1.21E-18 | ** |
| hop-miR36 | UCUGAAUUAUCAUUUAACAAA | 21 | 0.275741313 | 1.69728282 | 2.62183964 | 0.000117 | ** |
| hop-miR37 | CUAGUUUUCGGUACUGUAAAC | 21 | 0.137870657 | 5.971921033 | 5.436807747 | 8.66E-20 | ** |
| hop-miR38 | CACAUGAGCAAUAUAUUUGCA | 21 | 2.205930506 | 13.89257419 | 2.654854698 | 8.36E-31 | ** |
| hop-miR39 | UUUCUGUUCGACUGGUAGAGA | 21 | 4.687602326 | 67.38841418 | 3.845578487 | 1.63E-183 | ** |
| hop-miR40 | UCAGAAACUCCAUCGUCAACG | 21 | 26.60903673 | 130.3136032 | 2.291999502 | 8.69E-246 | ** |
| hop-miR41 | AUGUACAUUGUAGUUAUUUAGAG | 23 | 0.82722394 | 0.628623267 | −0.396082268 | 0.929292 | |
| hop-miR42 | UUUCUGAUCGACUGGUAGAGA | 21 | 0.275741313 | 15.46413236 | 5.809466644 | 1.19E-50 | ** |
| hop-miR43 | CGGGGCUUUGGGUUCAUCACC | 21 | 4.549731669 | 9.115037366 | 1.002467109 | 2.19E-09 | |
| hop-miR44 | UGACAGUUUGAUCAUAUUGAC | 21 | 0.01 | 5.84619638 | 9.191354481 | 6.08E-21 | ** |
| hop-miR45 | AGUUUAGGGGCCUAUUUGAACU | 22 | 0.137870657 | 0.691485593 | 2.326383757 | 0.027589 | ** |
| hop-miR46 | UCAAACCGUCCAAACCACACCGU | 23 | 0.01 | 1.50869584 | 7.237158171 | 6.99E-06 | ** |
| hop-miR47 | CCCUAGACUUAGAUUUGAU | 19 | 0.275741313 | 0.314311633 | 0.188880233 | 0.651715 | |
| hopmiR48 | GGGAGACUUUGUAGACAUUCAAC | 23 | 0.01 | 0.251449307 | 4.65219567 | 0.162062 | ** |
| hop-miR49 | CGUGGAAAACAUGUUUGAACCU | 22 | 1.102965253 | 8.360689446 | 2.922234574 | 2.47E-20 | ** |