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. 2016 Nov 15;16:86. doi: 10.1186/s12935-016-0361-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Knockdown of α-synuclein (α-Syn) reduces cell proliferation and colony formation and increases apoptosis in meningioma cells. a Measurement of α-Syn mRNA and protein levels in IOMM-Lee meningioma cells transfected with control or α-Syn shRNA. b BrdU incorporation assay was performed to determine cell proliferation in IOMM-Lee cells transfected with control or α-Syn shRNA. c Colony formation assay. IOMM-Lee cells transfected with control or α-Syn shRNA were allowed to form colonies for 10 days. Top representative dishes. Bottom quantification of colony formation. d Apoptosis was quantified by flow cytometry. Top representative dot plots of cells stained with Annexin-V and PI. Bottom quantitative data from three independent experiments. *P < 0.05 versus cells transfected with control shRNA