Fig. 5.

Overexpression of α-synuclein (α-Syn) promotes CH-157MN cell growth and invasion. a Western blot analysis of α-Syn protein levels in CH-157MN cells transfected with vector (control) or α-Syn-expressing plasmid. Top representative Western blots from three independent experiments. Bottom quantitative analysis of the α-Syn protein levels. b BrdU incorporation assay was performed to determine cell proliferation in CH-157MN cells. c Colony formation assay. CH-157MN cells transfected with vector or α-Syn-expressing plasmid were allowed to form colonies for 10 days. Top representative dishes. Bottom quantification of colony formation. d Transwell invasion assay. Top representative images of cells that invaded through the Matrigel-coated insert. Bottom quantitative data from three independent experiments. *P < 0.05 versus cells transfected with vector