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. 2016 Nov 14;15:194. doi: 10.1186/s12934-016-0589-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Analysis of ATF-SAP expression in Pichia pastoris after codon usage optimization of the ATF domain. Histograms showing the distribution of 14 (ATFopt-SAP, dark blue) and 15 (ATF-SAP, light blue) independent clones induced for 24 h for expression of native or optimized (opt) ATF-SAP. Expression levels are reported in different ranges from the lowest (1.00–1.99 mg/l) to the highest expressers (6.00–6.99 mg/l)