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. 2016 Nov 14;15:194. doi: 10.1186/s12934-016-0589-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Production of ATF-SAPs in bioreactor. Pichia pastoris growth curve (a), glycerol (b) and methanol (c) concentration were monitored before and after the induction of ATF-SAP WT (blue line) or ATF-SAP KQ (red line) expression. The light blue arrows indicate the pulses of methanol. Error bars represent standard deviations from the mean of triplicate samples. The chimeric fusion proteins secreted into the supernatants at the indicated time points were analyzed by western blotting using an anti-saporin antibody as probe (d). Bands were quantified and expressed as fold increase (e)