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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Virology. 2016 Sep 22;499:178–184. doi: 10.1016/j.virol.2016.09.005

Figure 2. Interaction of E1A 1-80 and E1A 243R with NuA4 components through the TRRAP targeting domain.

Figure 2

A. Illustration of expression constructs. B. Adenoviral-expressed E1A 1-80FH interacts with components of the NuA4 complex identified by proteomic analysis. HeLa cells were infected with Ad expression vectors as indicated, and cell lysates co-immunoprecipitated with Flag antibody beads. Bound proteins were identified by Western blots with indicated antibodies. E1A Western blot analysis was with a rabbit polyclonal antibody. The apparent low quantity of E1A Δ2-11FH in the E1A Western blot analysis was possibly due to weak binding of the protein to the PVDF membrane during the assay. E1A Western blot analysis with Flag and HA antibodies revealed the same result (not shown). C. Adenoviral-expressed E1A 243R interacts with the identified components of the NuA4 complex. HeLa cells were infected with Ad-lacZ (lane 1) or Ad-E1A 243R (lane 2), and co-immunoprecipitation was with E1A M73 antibody beads (SCBT, Inc.). Western blot analysis was as in B, except E1A 243R Western blot analysis was with M58 antibody (SCBT, Inc.)