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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: Science. 2016 Sep 30;353(6307):1549–1552. doi: 10.1126/science.aaf7463

Fig. 4. The cyclin A2 C terminus directly binds to a conserved sequence in the Mre11 3′UTR.

Fig. 4

(A) RNA immunoprecipitation in wild-type MEFs expressing the indicated HA-tagged cyclin A2 deletion mutants. RNAs coprecipitating with HA–cyclin A2 mutants were analyzed by RT-qPCR. (B) Quantification of cyclin A2302–432–expressing cells with two or more γH2AX- and 53BP1-colocalized foci. (C) Luciferase activity in MEFs transfected with different regions of the Mre11 3′UTR. (D) Representative native gel shifts showing a specific interaction between recombinant cyclin A2302–432 and the Mre11 3′UTR conserved sequence. (E) Same as (D) but in the presence of recombinant human eIF4A2. (F) Western blot analysis of eIF4A2-coprecipitating proteins in wild-type MEFs. Data in (A) to (C) are means ± SEM (N = 3 independent MEF lines per genotype). *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired t test).