IN administration of Lip-LcrV L. plantarum is not protective. a, LcrV specific IgG1, IgG2a, IgG2b, and IgG3 levels following vaccination and boost with Lip-LcrV L. plantarum (Lip-LcrV) (n=5), empty vector (n=5), rLcrV in alum (rLcrV IM) (n=5), or unvaccinated (negative) (n=5). b, 50% maximal binding titers were calculated for the indicated LcrV specific isotypes in Lip-LcrV and rLcrV IM vaccinated mice. Titers could not be generated empty vector or negative mice. c, LcrV specific IgA in BAL washes of vaccinated and control mice. d, survival of mice after challenge with 10× LD50 of Y. pestis CO92pgm−. #-p<0.01 rLcrV IM vs. Empty Vector, Lip-LcrV, rLcrV IM survival. These data are representative of five experiments.