Figure 3. Increasing levels of apoptosis (annexin V binding) in rested HIV-specific CD8⁺ T cells.

CD8⁺ T cells were isolated and rested in vitro for 0–3 d, stained with 1 class I HIV-specific tetramer and with annexin V, and measured by polychromatic flow cytometry. Late apoptotic/necrotic cells were excluded by gating out cells that were positive for the dead cell dye 7AAD. (A) Annexin V binding by the total CD8 population. (B) Annexin V binding by the HIV-specific CD8 population (tetramer-positive cells). ECs (circles), CPs (squares). (C) Kinetics of Annexin V binding over 3 d of in vitro incubation in the HIV-specific and total CD8 population.