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. 2016 Jul 12;100(6):1425–1433. doi: 10.1189/jlb.4A0915-422R

Figure 4. Preferential loss of effector functions and effector memory cells in rested CD8+ T cells from CPs compared to ECs.

Figure 4.

(A) CD8+ T cells from EC and CP (freshly thawed or rested) were cocultured with Gag-pulsed autologous CD4s. Degranulation (CD107a) and 5 effector functions (production of IFN-γ, MIP-1β, TNFα, perforin, and granzyme B) were measured by polychromatic flow cytometry. Background levels (measured under no antigen stimulation) were subtracted. Granzyme B and perforin were quantified gating on HIV-specific CD8+ T cells (IFN-γ+). Fold difference: freshly thawed vs. rested. (B) Unstimulated CD8+ T cells from 7 ECs and 7 CPs were stained for CD45RA, CD27, and CCR7 for memory subset profiling. Fold difference: freshly thawed vs. rested CD8+ T cells.