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. 2016 Jul 21;100(6):1395–1404. doi: 10.1189/jlb.3A0316-125R

Figure 4. Dex suppresses NFκB and AP-1 transcriptional activity.

Figure 4.

(A–D) BMMCs were left unactivated (0 min) or activated with IL-33 (100 ng/ml) plus simultaneous addition of vehicle or Dex (2 µM). Lysates collected at 5 and 15 min after activation were used for Western blot analysis. Normalized signals for individual time points were plotted vs. untreated cells. Data are representative of 2 independent experiments performed in triplicate. (E) Transfected BMMCs were activated with IL-33 (50 ng/ml) and simultaneously treated with vehicle or Dex (2 μM) for 2 h. Ratios of signal for firefly luciferase to that of Renilla luciferase for individual samples were normalized to that of vehicle-treated cells. Data are the average of 2 independent experiments performed in triplicate. Tukey’s multiple-comparison test was used to determine the P values. *P < 0.05; ***P < 0.001; ****P < 0.0001.